Area of Clinical Practice
“Skeletal Muscle Weakness and Thermogenesis in Human Endotoxaemia”
This thesis was undertaken under the supervision of Professor Gordon L Carlson (Injury Research Group, Hope Hospital, Salford) and Dr Judith A Hoyland (Department of Laboratory and Regenerative Medicine, University of Manchester). I formulated the research hypothesis, and planned the experimental work. The two-year research period was fully funded by research fellowships and bursaries totalling £189,000, awarded to me personally, in open national competition.
Aim- to clarify the mechanisms that lead to muscle weakness and thermogenesis in a human endotoxaemia model of sepsis.
1) that acute endotoxaemia would lead to impaired contractile function and fibre specific induction of pro-inflammatory cytokine expression (particularly TNF-?) within skeletal muscle.
2) that the fever and increased thermogenesis associated with administration of endotoxin in humans would be associated with up-regulation of uncoupling protein gene expression in human skeletal muscle.
Laboratory research- techniques employed were radioactive in situ hybridisation, immuno-histochemistry and both standard PCR and Real Time PCR.
Courses attended: modular teaching courses in scientific methods, core skills and data handling within the University of Manchester, including “Analysing Data; Introduction to SPSS” and the BJS sponsored “How to write a Clinical Paper”, as well as external courses: “Research Methods for Surgeons” and “Finding the Evidence” at the Royal College of Surgeons of England.
MD awarded July 2006.
This work was presented at The Physiology Society and the Society of Academic & Research Surgery national meetings and the results published in the British Journal of Surgery.
Associate fellow of Royal College of Anaesthetist. ESA member. Spanish Society of Anaesthesia member.
Anaesthesiology training in Spain. Consultant Anaesthetist in Manacor and La Fe Hospitals. Consultant in this trust from 2004
LMS, Specialist in Anaesthesia.
Spanish program for specialists in Anaesthesiology